Histomorphometrical, Histochemical and Electron Microscopic Studies of Goblet Mucous Cells in Different Regions of Argyrosomus hololepidotus Epidermis
2018
Morovvati, Hassan | Esfandiyari, Kaveh | Anbara, Hojat
BACKGROUND: Skin is the first line of defense against the external environment and and it is possible to maintain the natural physiological functions in the body. The mucus layer on the surface of the fish body contains anti-microbial combination that provides the first layer of defense against pathogens. The mucus is released by some of the epidermis cells which are called goblet cells and it mostly contains the mucin and other glycoproteins. OBJECTIVES: Histomorphometrical, Histochemical and Electron Microscopic Studies of Goblet Mucous Cells in Different Regions of Argyrosomus Hololepidotus Epidermis. METHODS: In this study, six Argyrosomus hololepidotus are used and the structure of the fish’s skin was studied. For doing this microscopic study, the sampling was done on dorsal Regions of fish with a thickness of 0.5µ then they were stained with H & E, PAS, AB (PH = 2.5) and AB (PH = 2.5)-PAS. For electron microscopic study, the samples after primary and post-fixation were dehydrated and were embedded in resin. Then, thin sections 50 μm were prepared and stained with uranyl acetate. RESULTS: Argyrosomus hololepidotus fish has maximum goblet cells in ventral and dorsal skin and minimum numbers of goblet cells were seen in tail skin in 100 µm length of epidermis. There were goblet mucous cells containing mucous in the Argyrosomus Hololepidotus epidermal that thier numbers were different in difference areas but mucus components were similar in different areas and they reacted positively to PAS and AB dyes with PH = 2.5.The electron microscopic results of this study were showed that goblet cells immigrate in thickness of epidermis and they include mucosal drops. CONCLUSIONS: There are goblet mucus cells in all parts of Argyrosomus Hololepidotus Epidermis and they have similar mucus nature.
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