Identificación de fitocompuestos antioxidantes en el extracto acuoso de Phyllanthus orbicularis Kunth | Fractionation of an aqueous extract of Phyllanthus orbicularis Kunth and identification of antioxidant compounds
2023
Ángel Sánchez-Lamar | José A. Pérez | Josep Ros | Josefina Pons | Mirle Ferrer | Jorge L. Fuentes | Montserrat Llagostera
espagnol; castillan. The aqueous extract of Phyllanthus orbicularis contains bioproducts able tosignificantly reduce the mutagenesis mediated by oxidative compounds. Abioactive-directed fractionation of the aqueous extract of P. orbicularis hasbeen applied with the aim of identifying antimutagenic fractions, which al-lowed isolating and identifying some biocompound responsible for this activ-ity. Antimutagenesis of all fractions was determined by the Salmonellaretromutation assay, using hydrogen peroxide as model mutagen. Applyingthis methodology, two potent antimutagenic fractions have been obtainedable to totally abolish the mutagenicity produced by hydrogen peroxide. Inthese fractions 2,4-di-tertbutylphenol, OO’-diphenol-4,4’,6,6’-tetra-tert-butyland 2,6-di-sec-butylphenol were identified by Gas Chromatography/MassSpectrometry
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