Cytogenomic Characterization of Transposable Elements and Satellite DNA in Passiflora L. Species
2024
Silva, Gonçalo, Santos | Souza, Margarete, Magalhães | Pamponét, Vanessa, de Carvalho Cayres | Micheli, Fabienne | Melo, Cláusio, Antônio Ferreira De | Oliveira, Sárah, Gomes De | Costa, Eduardo, Almeida | Universidade Estadual De Santa Cruz [Brazil] (UESC) | Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier ; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM) | Département Systèmes Biologiques (Cirad-BIOS) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) | Universidade de São Paulo = University of São Paulo (USP) | Instituto de Biociencias ; Universidade de São Paulo = University of São Paulo (USP) | This research was funded by FAPESB (Fundação de Amparo à Pesquisa do Estado da Bahia) and CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior), grant number 1987/2–13. The APC was funded by UESC (Universidade Estadual de Santa Cruz).
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Afficher plus [+] Moins [-]anglais. The species Passiflora alata, P. cincinnata, and P. edulis have great economic value due to the use of their fruits for human consumption. In this study, we compared the repetitive genome fractions of these three species. The compositions of the repetitive DNA of these three species' genomes were analyzed using clustering and identification of the repetitive sequences with RepeatExplorer. It was found that repetitive DNA content represents 74.70%, 66.86%, and 62.24% of the genome of P. alata, P. edulis, and P. cincinnata, respectively. LTR Ty3/Gypsy retrotransposons represent the highest genome proportions in P. alata and P. edulis, while Ty1/Copia comprises the largest proportion of P. cincinnata genome. Chromosomal mapping by Fluorescent In Situ Hybridization (FISH) showed that LTR retrotransposons have a dispersed distribution along chromosomes. The subtelomeric region of chromosomes is where 145 bp satellite DNA is located, suggesting that these elements may play important roles in genome structure and organization in these species. In this work, we obtained the first global characterization of the composition of repetitive DNA in Passiflora, showing that an increase in genome size is related to an increase in repetitive DNA, which represents an important evolutionary route for these species.
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