Quantitative MRI imaging of parenchyma and venation networks in Brassica napus leaves: effects of development and dehydration
2024
Boulc'h, Pierre-Nicolas | Collewet, Guylaine | Guillon, Baptiste | Quellec, Stephane | Leport, Laurent | Musse, Maja | Optimisation des procédés en Agriculture, Agroalimentaire et Environnement (UR OPAALE) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Institut de Génétique, Environnement et Protection des Plantes (IGEPP) ; Université de Rennes (UR)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Rennes Angers ; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) | This work was supported by a PhD grant to Pierre-Nicolas Boulc’h from the Regional Council of Bretagne and the University of Rennes.
International audience
Afficher plus [+] Moins [-]anglais. BackgroundCharacterisation of the structure and water status of leaf tissues is essential to the understanding of leaf hydraulic functioning under optimal and stressed conditions. Magnetic Resonance Imaging is unique in its capacity to access this information in a spatially resolved, non-invasive and non-destructive way. The purpose of this study was to develop an original approach based on transverse relaxation mapping by Magnetic Resonance Imaging for the detection of changes in water status and distribution at cell and tissue levels in Brassica napus leaves during blade development and dehydration.ResultsBy combining transverse relaxation maps with a classification scheme, we were able to distinguish specific zones of areoles and veins. The tissue heterogeneity observed in young leaves still occurred in mature and senescent leaves, but with different distributions of T2 values in accordance with the basipetal progression of leaf blade development, revealing changes in tissue structure. When subjected to severe water stress, all blade zones showed similar behaviours.ConclusionThis study demonstrates the great potential of Magnetic Resonance Imaging in assessing information on the structure and water status of leaves. The feasibility of in planta leaf measurements was demonstrated, opening up many opportunities for the investigation of leaf structure and hydraulic functioning during development and/or in response to abiotic stresses.
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