Effects of benzyl amino purine (BAP) different concentation for shoot multiplication of Nephentes ampullaria Jack
2021
Nazirah Abdullah | Nor Hasnida Hassan | Rozidah Khalid | Sabariah Ramuddin | Muhammad Fuad Yahya | Rohani Abdullah | Mohd Saifuldullah Abd. Wahid
Nephentes ampullaria Jack is a carnivorous plant that can be found in Malaysia, Borneo, the Maluku Islands, New Guinea, Sumatra, and Thailand. City development and human activity had affected the natural habitat of this unique plants. Pitchers of N. ampullaria commonly used for “Lemang periuk kera” in Malaysia. Although, the status of N. ampullaria in the Red List is Least Concern (LC), day by day, the plant getting difficult to be found. Tissue culture is an alternative way to propagate and plant it back to its habitat as well as for the production of its pitchers for lemang production. FRIM has made proactive initiative to produced tissue culture plants of N. ampularia for conservation and for commercial production of this species. Shoot multiplication medium played an important role to ensure the growth of healthy new shoots produced. In this study, different concentration of Benzyl amino purine (BAP) ranging from (0, 0.5, 1.0, 2.5 and 5.0 mg/L) in MS basal media were used as plant growth regulator for shoot multiplication of N. ampullaria. Cotyledonary seedlings were cultured in these medium and incubated in culture room at a temperature of 22.0 ± 2.0°C. After 8 weeks of culture, all cultures were observed and data obtained were analysed using the Duncan’s Multiple Range Test (DMRT). Results obtained showed that MS basal media added with 2.5 mg/L BAP is the optimal medium for shoot multiplication of N. ampularia with the highest mean number of new shoots (2.8 ± 0.26 ) compared to others.
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