The effect of optimum dosage of Ovaprim injection on artificial spawning of Esox lucius
2014
Khaval, A. | Dezhandiyan, S. | Mahisefat, F. | Amiri, A. | Sharifian, M.
This project was conducted to explore the effect of optimum dosage of Ovaprim injection on artificial spawning of Esox lucius. The research implemented by 4 treatments with 3 replicates for each ones. Three female and six male brooders were injected in each replicate. The animals in 1, 2 and 3 treatments were injected by 10, 20 and 30 µg/kg BW, respectively, and 4 th treatment was considered as the control being injected with 4 mg/kg BW pituitary gland extract. Average (±SE) weights were 1361±521, 1376±954, 1009±160 and 1100 ±422 g in 1, 2, 3 and 4 treatments in females, respectively. In addition, positive response percent to hormone injection were measured as 77.8 ±19.24, 88.9 ± 19.24, 55.5 ±50.91 and 55.5 ± 19.24 % in 1, 2, 3 and 4 treatments in female and 94.4 ± 9.58, 88.9 ±19.26, 83.3±28.86 and 88.9 ± 19.26 % in male brooders, respectively. However, there was no significant different between all treatments. Fertilization content (±SE) in one to four treatments measured as 87.1±10, 88.04±7.7, 83.9±5.2 and 72.4±19.7 %, respectively. No significant differences were found among pairwise treatments. Average (±SE) percentage of eyed eggs were 66.6±15.9 for treatment one, 61.2±22.3 in treatment two, 58.3±10.7 in treatment three, and 56.1±15.04 in treatment four, with no significant pairwise differences. The average (±SE) hatching eggs were measured as 27.41±19.8 in treatment one, 39.53±26.9 in treatment two, 95.18±5.6 in treatment three and 26.78±12.4 in treatment four, with no significane pairwise differences. Also, the best dosage injection of ovaprim hormone was 10 and 20 µg/kgBW.
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