Multi‐scale characterisation of cold response reveals immediate and long‐term impacts on cell physiology up to seed composition in sunflower
2025
Leconte, Jean Michel Louis | Moroldo, Marco | Blanchet, Nicolas | Bindea, Gabriela | Carrere, Sébastien | Catrice, Olivier | Comar, Alexis | Labadie, Marc | Marandel, Rémy | Pouilly, Nicolas | Tapy, Camille | Paris, Clémence | Mirleau‐thébaud, Virginie | Langlade, Nicolas Bernard | Laboratoire des Interactions Plantes Microbes Environnement (LIPME) ; Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Syngenta France | Agroécologie et phénotypage des cultures (APC) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)) ; École Pratique des Hautes Études (EPHE) ; Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université Paris Cité (UPCité) | Hiphen | ANR-11-INBS-0012,PHENOME,Centre français de phénomique végétale(2011) | ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010) | ANR-11-IDEX-0002,UNITI,Université Fédérale de Toulouse(2011)
International audience
Afficher plus [+] Moins [-]anglais. Early sowing can help summer crops escape drought and can mitigate the impacts of climate change on them. However, it exposes them to cold stress during initial developmental stages, which has both immediate and long‐term effects on development and physiology. To understand how early night‐chilling stress impacts plant development and yield, we studied the reference sunflower line XRQ under controlled, semi‐controlled and field conditions. We performed high‐throughput imaging of the whole plant parts and obtained physiological and transcriptomic data from leaves, hypocotyls and roots. We observed morphological reductions in early stages under field and controlled conditions, with a decrease in root development, an increase in reactive oxygen species content in leaves and changes in lipid composition in hypocotyls. A long‐term increase in leaf chlorophyll suggests a stress memory mechanism that was supported by transcriptomic induction of histone coding genes. We highlighted DEGs related to cold acclimation such as chaperone, heat shock and late embryogenesis abundant proteins. We identified genes in hypocotyls involved in lipid, cutin, suberin and phenylalanine ammonia lyase biosynthesis and ROS scavenging. This comprehensive study describes new phenotyping methods and candidate genes to understand phenotypic plasticity better in response to chilling and study stress memory in sunflower.
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