Mutations in the Arabidopsis rol17/<em>isopropylmalate synthase 1</em> locus alter amino acid content, modify the tor network, and suppress the root hair cell development mutant <em>lrx1</em>
2019
Schaufelberger, Myriam | Galbier, Florian | Herger, Aline | Francisco, Rita de Brito | Roffler, Stefan | Clément, Gilles, G. | Diet, Anouck | Hortensteiner, Stefan | Wicker, Thomas | Ringli, Christoph | Universität Zürich [Zürich] = University of Zurich (UZH) | Zurich Basel Plant Science Center ; Universität Zürich [Zürich] = University of Zurich (UZH)-Université de Bâle = University of Basel = Basel Universität (Unibas) | Institut Jean-Pierre Bourgin (IJPB) ; Institut National de la Recherche Agronomique (INRA)-AgroParisTech | Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)) ; Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS) | LabEx Saclay Plant Sciences-SPS [ANR-10-LABX-0040-SPS]; PSC - Syngenta Research Fellowship Program; Swiss National Science FoundationSwiss National Science Foundation (SNSF) [31003A_166577/1] | ANR-17-EURE-0007,SPS-GSR,Ecole Universitaire de Recherche de Sciences des Plantes de Paris-Saclay(2017) | ANR-10-LABX-0040,SPS,Saclay Plant Sciences(2010)
International audience
Afficher plus [+] Moins [-]anglais. The growth and development of organisms must be tightly controlled and adjusted to nutrient availability and metabolic activities. The Target of Rapamycin (TOR) network is a major control mechanism in eukaryotes and influences processes such as translation, mitochondrial activity, production of reactive oxygen species, and the cytoskeleton. In Arabidopsis thaliana, inhibition of the TOR kinase causes changes in cell wall architecture and suppression of phenotypic defects of the cell wall formation mutant lrx1 (leucine-rich repeat extensin 1). The rol17 (repressor of lrx1 17) mutant was identified as a new suppressor of lrx1 that induces also a short root phenotype. The ROL17 locus encodes isopropylmalate synthase 1, a protein involved in leucine biosynthesis. Dependent on growth conditions, mutations in ROL17 do not necessarily alter the level of leucine, but always cause development of the rol17 mutant phenotypes, suggesting that the mutation does not only influence leucine biosynthesis. Changes in the metabolome of rol17 mutants are also found in plants with inhibited TOR kinase activity. Furthermore, rol17 mutants show reduced sensitivity to the TOR kinase inhibitor AZD-8055, indicating a modified TOR network. Together, these data suggest that suppression of lrx1 by rol17 is the result of an alteration of the TOR network.
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