TILLING by Sequencing (TbyS) for targeted genome mutagenesis in crops
2017
Kumar, Anishkumar P. K. | Mckeown, Peter C. | Boualem, Adnane | Ryder, Peter | Brychkova, Galina | Bendahmane, Abdelhafid | Sarkar, Abhimanyu | Chatterjee, Manash | Spillane, Charles | Genetics and Biotechnology Lab, Plant & AgriBiosciences Research Centre (PABC), School of Natural Sciences ; National University of Ireland [Galway] (NUI Galway) | Bench Bio Private Limited (BenchBio) | Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)) ; Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS) | Metabolic Biology Department ; John Innes Centre [Norwich] ; Biotechnology and Biological Sciences Research Council (BBSRC)-Biotechnology and Biological Sciences Research Council (BBSRC) | Science Foundation Ireland (SFI) (02/IN.1/B49 ; 08/IN.1/B1931)
TILLING (Targeting Induced Local Lesions in Genomes) by Sequencing (TbyS) refers to the application of high-throughput sequencing technologies to mutagenised TILLING populations as a tool for functional genomics. TbyS can be used to identify and characterise induced variation in genes (controlling traits of interest) within large mutant populations, and is a powerful approach for the study and harnessing of genetic variation in crop breeding programmes. The extension of existing TILLING platforms by TbyS will accelerate crop functional genomics studies, in concert with the rapid increase in genome editing capabilities and the number and quality of sequenced crop plant genomes. In this mini-review, we provide an overview of the growth of TbyS and its potential applications to crop molecular breeding.
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