Expanding the tools for identifying mononuclear phagocyte subsets in swine: Reagents to porcine CD11c and XCR1 | Accroitre les outils pour identifier les sous-populations de phagocytes mononucléés chez le porc : Réactifs contre CD11c et XCR1
2016
Deloizy, Charlotte | Bouguyon, Edwige | Fossum, Even | Sebo, Peter | Osicka, Radim | Bole, Angelique | Pierres, Michel | Biacchesi, Stephane | Dalod, Marc | Bogen, Bjarne | Bertho, Nicolas | Schwartz-Cornil, Isabelle | Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)) ; Institut National de la Recherche Agronomique (INRA) | University of Oslo (UiO) | Institute of Microbiology of the Czech Academy of Sciences [Prague, Czech Republic] (MBU / CAS) ; Czech Academy of Sciences [Prague] (CAS) | Mi-mAbs (C/O CIML) ; Centre d'Immunologie de Marseille - Luminy (CIML) ; Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) | Centre d'Immunologie de Marseille - Luminy (CIML) ; Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
International audience
Afficher plus [+] Moins [-]anglais. Pig is a domestic species of major importance in the agro-economy and in biomedical research. Mononuclear phagocytes (MNP) are organized in subsets with specialized roles in the orchestration of the immune response and new tools are awaited to improve MNP subset identification in the pig. We cloned pig CD11c cDNA and generated a monoclonal antibody to pig CD11c which showed a pattern of expression by blood and skin MNP subsets similar to humans. We also developed a porcine XCL1-mCherry dimer which specifically reacted with the XCR1-expressing dendritic cell subset of the type 1 lineage in blood and skin. These original reagents will allow the efficient identification of pig MNP subsets to study their role in physiological and pathological processes and also to target these cells in novel intervention and vaccine strategies for veterinary applications and preclinical evaluations. (C) 2016 Elsevier Ltd. All rights reserved.
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