Expansion of the Strigolactone Profluorescent Probes Repertory: The Right Probe for the Right Application
2022
de Saint Germain, Alexandre | Clavé, Guillaume | Schouveiler, Paul | Pillot, Jean-Paul | Singh, Abhay-Veer | Chevalier, Arnaud | Daignan Fornier, Suzanne | Guillory, Ambre | Bonhomme, Sandrine | Rameau, Catherine | Boyer, François-Didier | Institut Jean-Pierre Bourgin - Sciences du végétal (IJPB) ; AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Institut de Chimie des Substances Naturelles (ICSN) ; Institut de Chimie - CNRS Chimie (INC-CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS) | The CHARM3AT LabEx program (ANR-11-LABX-39) is also acknowledged for its support. | ANR-12-BSV6-0004,StrigoPath,Voies de signalisation des strigolactones (et/ou de dérivés) chez les plantes terrestres(2012) | ANR-21-CE20-0026,STIGO,Contrôle de l'orobanche du tournesol: identification des stimulants de germination chez l'hôte et récepteur(s) chez l'orobanche(2021) | ANR-17-EURE-0007,SPS-GSR,Ecole Universitaire de Recherche de Sciences des Plantes de Paris-Saclay(2017) | ANR-05-BDIV-0006,NiceFigs,Nouvelles recherches sur l'écologie des communautés : apports du modèle Ficus(2005) | European Project: 267196,EC:FP7:PEOPLE,FP7-PEOPLE-2010-COFUND,AGREENSKILLS(2012)
International audience
Afficher plus [+] Moins [-]anglais. Strigolactones (SLs) are intriguing phytohormones that not only regulate plant development and architecture but also interact with other organisms in the rhizosphere as root parasitic plants (Striga, Orobanche, and Phelipanche) and arbuscular mycorrhizal fungi. Starting with a pioneering work in 2003 for the isolation and identification of the SL receptor in parasitic weeds, fluorescence labeling of analogs has proven a major strategy to gain knowledge in SL perception and signaling. Here, we present novel chemical tools for understanding the SL perception based on the enzymatic properties of SL receptors. We designed different profluorescent SL Guillaume Clavé (GC) probes and performed structure-activity relationship studies on pea, Arabidopsis thaliana, and Physcomitrium (formerly Physcomitrella) patens. The binding of the GC probes to PsD14/RMS3, AtD14, and OsD14 proteins was tested. We demonstrated that coumarin-based profluorescent probes were highly bioactive and well-adapted to dissect the enzymatic properties of SL receptors in pea and a resorufin profluorescent probe in moss, contrary to the commercially available fluorescein profluorescent probe, Yoshimulactone Green (YLG). These probes offer novel opportunities for the studies of SL in various plants.
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