A modified method for rapid tryptophan analysis of maize
1969
Hernandez H., H. | Bates, L.S.
Due to their low lysine content, the cereal grains are generally considered of reduced nutritional quality in comparison to other protein sources. In maize, a~ second diet limiting amino acid is tryptophan. That increases in both of these amino acids are needed to improve the nutritional quality of maize has been known for many years. With the demonstrated improvement possible with opaque-2 maize, breeders are paying growing attention to improving nutritional quality through increasing the content of these two amino acids. However, the success of such research efforts depends ultimately upon the rapidity with which one can determine the lysine and/or the tryptophan content of individual plants or seeds in segregating populations. From the observed relationship between lysine and tryptophan in the zein fraction and its relationship to the whole endosperm, it was believed a tryptophan value could serve as a single parameter for maize evaluation. This study demonstrates the feasibility of rapid tryptophan analys s and the magnitude of the correlation between. tryptophan and lysine.
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