Insights into the Host Range, Genetic Diversity, and Geographical Distribution of Jingmenviruses
2019
Temmam, Sarah | Bigot, Thomas | Chrétien, Delphine | Gondard, Mathilde | Pérot, Philippe | Pommelet, Virginie | Dufour, Evelyne | Petres, Stéphane | Devillers, Elodie | Hoem, Thavry | Pinarello, Valérie | Hul, Vibol | Vongphayloth, Khamsing | Hertz, Jeffrey, C | Loiseau, Irène | Dumarest, Marine | Duong, Veasna | Vayssier Taussat, Muriel | Grandadam, Marc | Albina, Emmanuel | Dussart, Philippe | Moutailler, Sara | Cappelle, Julien | Brey, Paul, T | Eloit, Marc | Découverte de Pathogènes - Pathogen Discovery ; Biologie des Infections - Biology of Infection ; Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM) | Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB ; Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS) | Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA) | Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR) ; École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie ; Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12) | Institut Pasteur du Laos ; Pasteur Network (Réseau International des Instituts Pasteur) | Plateforme technologique Production et purification de protéines recombinantes – Production and Purification of Recombinant Proteins Technological Platform (PPR) ; Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS) | Institut Pasteur du Cambodge ; Pasteur Network (Réseau International des Instituts Pasteur) | US Naval Medical Research Unit n°2 | Unité Mixte de Recherche d'Épidémiologie des maladies Animales et zoonotiques (UMR EPIA) ; Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS) | École nationale vétérinaire d'Alfort (ENVA) | Université Paris-Est (UPE) | This work was supported by Laboratoire d’Excellence (Integrative Biology of Emerging Infectious Diseases; grant no. ANR-10-LABX-62-IBEID) and by the Direction Internationale de l'Institut Pasteur. | We thank Jacques Bellalou and his team at the Production and Purification of Recombinant Proteins Technological Platform of Institut Pasteur for their help in expression of recombinant proteins; Yves Jacob and Mélanie Dos Santos for their technical assistance with the luminometer; the staff members at the ICAReB Platform of Institut Pasteur for access to human control sera; and all the people involved in the sampling of ticks, bats, and cattle in France, French Antilles, Lao PDR, and Cambodia. | ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010)
International audience
Afficher plus [+] Moins [-]anglais. Jingmenvirus is a recently identified group of segmented RNA viruses phylogenetically linked with unsegmented Flaviviridae viruses. Primarily identified in various tick genera originating in China, Jingmenvirus geographical distribution has rapidly expanded to cover Africa, South America, Caribbean, and Europe. The identification of Jingmen-related viruses in various mammals, including febrile humans, opens the possibility that Jingmenviruses may be novel tick-borne arboviruses. In this study, we aimed at increasing knowledge of the host range, genetic diversity, and geographical distribution of Jingmenviruses by reporting for the first time the identification of Jingmenviruses associated with Rhipicephalus microplus ticks originating in the French Antilles (Guadeloupe and Martinique islands), with Amblyomma testudinarium ticks in Lao PDR, and with Ixodes ricinus ticks in metropolitan France, and from urine of Pteropus lylei bats in Cambodia. Analyses of the relationships between the different Jingmenvirus genomes resulted in the identification of three main phylogenic subclades, each of them containing both tick-borne and mammal-borne strains, reinforcing the idea that Jingmenviruses may be considered as tick-borne arboviruses. Finally, we estimated the prevalence of Jingmenvirus-like infection using luciferase immunoprecipitation assay screening (LIPS) of asymptomatic humans and cattle highly exposed to tick bites. Among 70 French human, 153 Laotian human, and 200 Caribbean cattle sera tested, only one French human serum was found (slightly) positive, suggesting that the prevalence of Jingmenvirus human and cattle infections in these areas is probably low.
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