Improving bioavailability of phosphorous from cattle dung by using phosphatase immobilized on natural clay and nanoclay
2012
Calabi-Floody, Marcela | Velasquez, Gabriela | Gianfreda, Liliana | Saggar, Surinder | Bolan, Nanthi | Rumpel, Cornelia | Mora, Maria Luz | Universidad de la frontera [Tecumo, Chile] = University of La Frontera [Tecumo, Chile] = Université de La Frontera [Tecumo, Chili] (UFRO) | University of Naples Federico II = Università degli studi di Napoli Federico II (UNINA) | Manaaki Whenua – Landcare Research [Lincoln] | University of South Australia | Biogéochimie et écologie des milieux continentaux (Bioemco) ; École normale supérieure - Paris (ENS-PSL) ; Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS) | CONICYT (National foundation for Science and Technology) Chile [AT-240 80106]; FONDECYT [1100625]; MEC [80100008, 80100011]; ECOSSUD-CONICYT [C08U01]
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Afficher plus [+] Moins [-]anglais. The high P retention of acidic Andisols makes necessary to increase our technological approaches in pasture management in the animal system production. Here, we evaluated the clay- or nanoclay-acid phosphatase complexes for improving phosphorus mineralization from degraded cattle dung. We implemented an immobilization mechanism of acid phosphatase (AP) using natural clays (allophanic and montmorillonite) and nanoclays as support materials. Also, we evaluated the mineralization of organic P containing in decomposed cattle dung with clay- and nanoclay-AP complexes by incubation studies. Clays and nanoclays were characterized by microscopy techniques as atomic force and confocal-laser scanning microscopy. We found that these support materials stabilized AP by encapsulation. Our results showed that immobilization on allophanic or montmorillonite materials improved both the specific activity (4-48%) and the V-max (28-38%) of AP. Moreover, the enzyme had a better performance when immobilized on clay and nanoclay from Andisol than on montmorillonite materials. Phosphorous mineralization of cattle dung was regulated by water-soluble P present in the dung and P re-adsorption on allophanic materials. However, we were able to detect a potential capacity of AP immobilized on allophanic nanoclays as the best alternative for P mineralization. Further research with initially low water-soluble P containing organic materials is required to quantify the P mineralization potential and bioavailability of P from dung. (C) 2012 Elsevier Ltd. All rights reserved.
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