Experimental data from the development of Lymnaea stagnalis embryo test for chemicals hazard assessment
2024
Capela, Ricardo | Castro, Luís, Filipe | Santos, Miguel, Machado | Garric, Jeanne | Centro Interdisciplinar de Investigação Marinha e Ambiental = Interdisciplinary Centre of Marine and Environmental Research [Matosinhos, Portugal] (CIIMAR) ; Universidade do Porto = University of Porto | Universidade do Porto = University of Porto | RiverLy - Fonctionnement des hydrosystèmes (RiverLy) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
International audience
Afficher plus [+] Moins [-]anglais. This study aimed to contribute to the development of an embryo-test using the gastropod Lymnaea stagnalis , identified by the Organization for Economic Co-operation and Development (OECD) as a potential invertebrate test animal model. Together with the Potamopyrgus antipodarum , were the first mollusc models to be included in the organization testing guidelines. The focus was on validating an embryo toxicity test to cover the sensitive embryogenesis phase and on obtaining testing information on all of the model life cycle stages, contributing to close an identified gap within this context. Adhering to OECD guidelines, namely the L. stagnalis reproductive test, the study examined mortality rates, abnormality rates, development, growth, hatching rates, hearth rates, and pre-testing media suitability, during the embryogenesis, and the obtained dataset made available for further studies. Cadmium was chosen as the positive test compound due to its well-studied nature and the model's proven sensitivity to the compound, working as a reference compound for the test development.
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