Jasmonate inhibits adventitious root initiation through repression of CKX1 and activation of RAP2.6L transcription factor in Arabidopsis
2021
Dob, Asma | Lakehal, Abdellah | Novak, Ondrej | Bellini, Catherine | Umea Plant Science Centre, Department of Plant Physiology ; Umeå University = Umeå Universitet | Swedish University of Agricultural Sciences = Sveriges lantbruksuniversitet (SLU) | Umea Plant Science Center (UPSC) ; Department of Forest Genetics and Plant Physiology ; Swedish University of Agricultural Sciences = Sveriges lantbruksuniversitet (SLU)-Swedish University of Agricultural Sciences = Sveriges lantbruksuniversitet (SLU) | Umea Plant Science Centre, Department of Plant Physiology ; Umeå University = Umeå Universitet | Institut Jean-Pierre Bourgin - Sciences du végétal (IJPB) ; AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Swedish Research Council 2020-06430 | VINNOVA the Knut och Alice Wallenbergs Stiftelse | Carl Tryggers Stiftelse | Kempestiftelserna | Ministerstvo skolstvi, mladeze a telovychovy CZ. 02.1.01/0.0/0.0/16_019/0000827 | Grant Agency of the Czech Republic 19-00973S
International audience
Afficher plus [+] Moins [-]anglais. Abstract Adventitious rooting is a de novo organogenesis process that enables plants to propagate clonally and cope with environmental stresses. Adventitious root initiation (ARI) is controlled by interconnected transcriptional and hormonal networks, but there is little knowledge of the genetic and molecular programs orchestrating these networks. Thus, we have applied genome-wide transcriptome profiling to elucidate the profound transcriptional reprogramming events preceding ARI. These reprogramming events are associated with the downregulation of cytokinin (CK) signaling and response genes, which could be triggers for ARI. Interestingly, we found that CK free-base (iP, tZ, cZ and DHZ) content declined during ARI, due to downregulation of de novo CK biosynthesis and upregulation of CK inactivation pathways. We also found that MYC2-dependent jasmonate (JA) signaling inhibits ARI by downregulating the expression of the CYTOKININ OXIDASE/DEHYDROGENASE1 (CKX1) gene. We also demonstrated that JA and CK synergistically activate expression of RELATED to APETALA2.6 LIKE (RAP2.6L) transcription factor, and constitutive expression of this transcription factor strongly inhibits ARI. Collectively, our findings reveal that previously unknown genetic interactions between JA and CK play key roles in ARI
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