Air-water interfacial behaviour of whey protein and rapeseed oleosome mixtures
2021
Yang, Jack | Waardenburg, Leonie | Berton-Carabin, Claire | Nikiforidis, Constantinos | van Der Linden, Erik | Sagis, Leonard M.C. | Wageningen University and Research [Wageningen] (WUR) | Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Wageningen Food & Biobased Research ; Wageningen University and Research [Wageningen] (WUR) | The Netherlands Organisation for Scientific Research (NWO), and the Top Consortia for Knowledge and Innovation of the Dutch Min-istry of Economic Affairs (TKI). NWO project number:ALWTF.2016.001.
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Afficher plus [+] Moins [-]anglais. Hypothesis:Plant seeds store lipids in oleosomes, which are storage organelles with a triacylglycerol(TAG) core surrounded by a phospholipid monolayer and proteins. Due to their membrane components,oleosomes have an affinity for the air/oil–water interface. Therefore, it is expected that oleosomes canstabilise interfaces, and also compete with proteins for the air–water interface. Experiments:We mixedrapeseed oleosomes with whey protein isolate (WPI), and evaluated their air–water interfacial propertiesby interfacial rheology and microstructure imaging. To understand the contribution of the oleosome com-ponents to the interfacial properties, oleosome membrane components (phospholipids and membraneproteins) or rapeseed lecithin (phospholipids) were also mixed with WPI.Findings: Oleosomes werefound to disrupt after adsorption, and formed TAG/phospholipid-rich regions with membrane fragmentsat the interface, forming a weak and mobile interfacial layer. Mixing oleosomes with WPI resulted in aninterface with TAG/phospholipid-rich regions surrounded by whey protein clusters. Membrane components or lecithin mixed with proteins also resulted in an interface where WPI molecules aggregated intosmall WPI domains, surrounded by a continuous phase of membrane components or phospholipids. We also observed an increase in stiffness of the interfacial layer, due to the presence of oleosome membraneproteins at the interface.
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