Determination of metabolites of nitrofurans in chicken eggs by liquid chromatography with diode-array detector.
2023
Nitrofuran antibiotics have been banned for use in food-producing animals in many countries, including the European Union, owing to the threat they pose to human health. In the paper an improved liquid chromatography method using a diode-array detector, to detect parent compounds, furazolidone (AOZ), nitrofurantoin (AHD), furaltadone (AMOZ) and nitrofurazone (SEM), in chicken eggs is presented. Considering the requirement of MRPL (Minimum Required MRPL Performance Limit of 1 µg/kg), we achieved the required detection limit using high performance liquid chromatography by solid phase purification of the sample after extraction. For solid-phase purification, instead of expensive cartridges (columns), columns with silica gel of our own production were used. The method complies with the requirements of the European Commission Resolution 2002/657/ЕС. The validation of the method was conducted following the European Union criteria for the analysis of veterinary drug residues in foods. The decision limits (CCα) were 0.16-0.34 µg/kg, and the detection capabilities (CCβ) 0.20-0.35 µg/kg. The advantage of the method is that with relatively less financial costs it is possible to determine the amount less than the minimum working limit for nitrofuran metabolites set by the EU. Ref. 15.
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