Quantification of plasma reduced glutathione, oxidized glutathione and plasma total glutathione in healthy cats

Glutathione is an important intracellular tripeptide with multiple functions. Abnormal glutathione metabolism is thought to play an important role in various diseases of cats. However, no data regarding concentration of plasma glutathione are available for domestic cats. This study discusses the development of a rapid, simple high pressure liquid chromatography method for measurement of reduced glutathione (GSH), oxidized glutathione (GSSH) and total glutathione (GSHt) in plasma, for the purpose of establishing baseline data for future studies. The mean concentrations of GSH, GSSH and GSHt were 4.51 1 mM; 19.44 3.79 mM (expressed as GSH equivalent) and 23.59 3.89 mM, respectively. This is the first report of plasma glutathione mM; 19.44 3.79 mM (expressed as GSH equivalent) and 23.59 3.89 mM, respectively. This is the first report of plasma glutathione concentrations in this species.

Fil: Denzoin, Laura. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología; Argentina

Fil: Franci, Rodolfo J.. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Clínicas; Argentina

Fil: Tapia, Maria Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología; Argentina

Fil: Soraci, Alejandro Luis. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina