Spatially-explicit effects of small-scale clear-cutting on soil fungal communities in Pinus sylvestris stands

Clear-cutting is a common silvicultural practice. Although temporal changes in the soil fungal community after clear-cutting have been widely investigated, little is known about stand-level variations in the spatial distribution of soil fungi, particularly at the clear-cut edge. We performed spatial soil sampling in three clear-cuts (0.5 ha), edge habitats, and surrounding forests 8 years after clear-cutting to examine the impact of clear-cutting on the soil fungal community (diversity, composition, guilds, and biomass) and soil properties in a managed Pinus sylvestris forest in northern Spain. Our analyses showed small differences in the composition of the soil fungal community between edge, forest, and clear-cut zones, with <4 % of the species strictly associated with one or two zones. The richness, diversity, and evenness of the fungal community in the edge zone was not significantly different to that in the forest or clear-cut zones, although the clear-cut core had approximately a third fewer ectomycorrhizal species than the edge or the forest. Saprotrophic fungi were widespread across the clear-cut–forest gradient. Soil fungal biomass varied significantly between zones, ranging from 4 to 5 mg g−1 dry soil in the forest and at the forest edge to 1.7 mg g−1 dry soil in the clear-cut area. Soil organic matter, pH, nitrogen, and phosphorus did not differ significantly between edge, forest, and clear-cutting zones and were not significantly related to the fungal community composition. Overall, our study showed that small-scale clear-cut treatments are optimal to guarantee, in the medium-term, soil fungal communities within harvested areas and at the forest edge that are comparable to soil fungal communities in the forest, even though the amount of fungal biomass in the clear-cut zone is lower than at the forest edge or in the forest.

This work was partially supported by MicroEco - call Biodiversa+ 2021 ( PCI2022-135091-2 ), and by the Spanish Ministry of Science, Innovation and Universities and the European Social Fund ( RTI2018-099315-A-I00 ). This research was also supported by the project with grant number PID2022-139558OB-I00 funded by MCIN/AEI/10.13039/501100011033 and by “ ERDF A way of making Europe”. Giada Centenaro benefitted from Grant PRE2019-089112 funded by MCIN/AEI/10.13039/501100011033 and, as appropriate, by “ESF Investing in your future”. Josu G. Alday was supported by a Ramon y Cajal fellowship (RYC-2016-20528). José Antonio Bonet and Sergio de-Miguel benefitted from a Serra-Húnter Fellowship provided by the Generalitat of Catalonia.