Insights into the role of specific lipids in the formation and delivery of lipid microdomains to the plasma membrane of plant cells
2007
Laloi, Maryse | Perret, Anne-Marie | Chatre, Laurent | Melser, Su | Cantrel, Catherine | Vaultier, Marie-Noëlle | Zachowski, Alain | Bathany, Katell | Schmitter, Jean-Marie | Vallet, Myriam | Lessire, René | Hartmann, Marie-Andrée | Moreau, Patrick | Laboratoire de biogenèse membranaire (LBM) ; Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS) | Laboratoire de Génétique de la Levure ; Université de Poitiers = University of Poitiers (UP)-Centre National de la Recherche Scientifique (CNRS) | University of Saskatchewan [Saskatoon, Canada] (U of S) | Physiologie cellulaire et moléculaire des plantes (PCMP) ; Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) | Institut Européen de Chimie et de Biologie | Institut de Biologie Moléculaire des Plantes (IBMP) ; Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS) | This work was supported by the Centre National de la Recherche Scientifique (postdoctoral fellowship to M.L.), by the Victor Segalen University of Bordeaux 2, by the Conseil Re´gional d’Aquitaine (M.L., A.M.P., L.C., M.V., R.L., P.M.; mass spectrometry equipment to J.M.S.), and by the Pierre and Marie Curie University of Paris (C.C., M.N.V., A.Z.).
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Afficher plus [+] Moins [-]anglais. The existence of sphingolipid- and sterol-enriched microdomains, known as lipid rafts, in the plasma membrane (PM) of eukaryotic cells is well documented. To obtain more insight into the lipid molecular species required for the formation of microdomains in plants, we have isolated detergent (Triton X-100)-resistant membranes (DRMs) from the PM of Arabidopsis (Arabidopsis thaliana) and leek (Allium porrum) seedlings as well as from Arabidopsis cell cultures. Here, we show that all DRM preparations are enriched in sterols, sterylglucosides, and glucosylceramides (GluCer) and depleted in glycerophospholipids. The GluCer of DRMs from leek seedlings contain hydroxypalmitic acid. We investigated the role of sterols in DRM formation along the secretory pathway in leek seedlings. We present evidence for the presence of DRMs in both the PM and the Golgi apparatus but not in the endoplasmic reticulum. In leek seedlings treated with fenpropimorph, a sterol biosynthesis inhibitor, the usual D5-sterols are replaced by 9b,19-cyclopropylsterols. In these plants, sterols and hydroxypalmitic acid-containing GluCer do not reach the PM, and most DRMs are recovered from the Golgi apparatus, indicating that D5-sterols and GluCer play a crucial role in lipid microdomain formation and delivery to the PM. In addition, DRM formation in Arabidopsis cells is shown to depend on the unsaturation degree of fatty acyl chains as evidenced by the dramatic decrease in the amount of DRMs prepared from the Arabidopsis mutants, fad2 and Fad31, affected in their fatty acid desaturases.
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