Differences in hydrolytic enzyme activity accompany natural variation in mature aleurone morphology in barley (Hordeum vulgare L.)

Aubert, M.; Coventry, S.; Shirley, N.J.; Betts, N.; Würschum, T.; Burton, R.; Tucker, M.

Differences in hydrolytic enzyme activity accompany natural variation in mature aleurone morphology in barley (Hordeum vulgare L.)

2018

The aleurone is a critical component of the cereal seed and is located at the periphery of the starchy endosperm. During germination, the aleurone is responsible for releasing hydrolytic enzymes that degrade cell wall polysaccharides and starch granules, which is a key requirement for barley malt production. Inter- and intra-species differences in aleurone layer number have been identified in the cereals but the significance of this variation during seed development and germination remains unclear. In this study, natural variation in mature aleurone features was examined in a panel of 33 Hordeum vulgare (barley) genotypes. Differences were identified in the number of aleurone cell layers, the transverse thickness of the aleurone and the proportion of aleurone relative to starchy endosperm. In addition, variation was identified in the activity of hydrolytic enzymes that are associated with germination. Notably, activity of the free fraction of β-amylase (BMY), but not the bound fraction, was increased at grain maturity in barley varieties possessing more aleurone. Laser capture microdissection (LCM) and transcriptional profiling confirmed that HvBMY1 is the most abundant BMY gene in developing grain and accumulates in the aleurone during early stages of grain fill. The results reveal a link between molecular pathways influencing early aleurone development and increased levels of free β-amylase enzyme, potentially highlighting the aleurone as a repository of free β-amylase at grain maturity.

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Matthew K. Aubert, Stewart Coventry, Neil J. Shirley, Natalie S. Betts, Tobias Würschum, Rachel A. Burton and Matthew R. Tucker

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Mots clés AGROVOC

beta-amylase gene expression regulation hordeum hydrolysis plant

Informations bibliographiques

Editeur

Springer Nature Publishing

D'autres materias

Laser capture microdissection; Plant proteins

Langue

anglais

Format

application/pdf

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© The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

ISBN

11025-1-1025-

ISSN

2045-2322, 0000-0003, 2539-6980, 0000-0001, 8114-9891, 0000-0002, 3345-8160, 0638-4709, 4661-6700

Type

Journal Article; Journal Part

Source

https://doi.org/10.1038/s41598-018-29068-4

Sur AGRIS depuis: 2024-10-18

Date de modification: 2025-10-25

Format: Dublin Core

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Cette notice bibliographique a été fournie par University of Adelaide

Découvrez la collection de ce fournisseur de données dans AGRIS

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DOI http://hdl.handle.net/2440/117362

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Differences in hydrolytic enzyme activity accompany natural variation in mature aleurone morphology in barley (Hordeum vulgare L.)

2018

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Informations bibliographiques