Identification and purification of a purothionin homologue from rye (Secale cereale L.)
1978
Hernandez Lucas, Carlos | Carbonero Zalduegui, Pilar | García Olmedo, Francisco
Purothionins are high sulfur, basic polypeptides present in wheat endosperm, t h at can be extracted with petroleum-ether (in the form of lipid-protein complexes), and with dilute sulfuric acid. The extraction and purification of a purothionin homologue from rye kernels (Sécale cereale L.) is reported here for the first time; its cióse similarity to the /3-purothionin from wheat is demonstrated by its identical mobility in one- and two-dimensional electrophoresis and by its amino acid composition
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