Environmental dissemination of SARS-CoV-2 in a university hospital during the COVID-19 5th wave Delta variant peak in Castile-León, Spain

Producción Científica

The dominant SARS-CoV-2 Delta variant (B.1.617.2) became the main circulating variant among countries by mid 2021. Attention was raised to the increased risk of airborne transmission, leading to nosocomial outbreaks even among vaccinated individuals. Considering the increased number of COVID-19 hospital admissions fueled by the spread of the variant, with Spain showing the highest COVID-19 rates in mainland Europe by July 2021, the aim of this study was to assess SARS-CoV-2 environmental contamination in different areas of a University Hospital in the region of Castile-León, Spain, during the peak of the 5th wave of COVID-19 in the country (July 2021). Air samples were collected from sixteen different areas of the Hospital using a Coriolis® μ air sampler. Surface samples were collected in these same areas using sterile flocked plastic swabs. RNA extraction followed by a one-step RT-qPCR were performed for detection of SARS-CoV-2 RNA. Of the 21 air samples, only one was positive for SARS-CoV-2 RNA, from the emergency waiting room. Of the 40 surface samples, 2 were positive for SARS-CoV-2 RNA, both from the microbiology laboratory. These results may be relevant for risk assessment of nosocomial infection within healthcare facilities, thus helping prevent and minimize healthcare staff’s exposure to SARS-CoV-2, reinforcing the importance of always wearing appropriate and well-fit masks at all times and proper PPE when in contact with infected patients.

Fundación Portuguesa para la Ciencia y la Tecnología (FCT) - (projects projects UIDB/04750/2020 and LA/P/0064/2020)

Fundación para la Ciencia y la Tecnología/Ministerio de Ciencia,Tecnología y Educación Superior (FCT/MCTES, Portugal) - (projects LA/P/0045/2020 (ALiCE) and UIDB/00511/2020-UIDP/00511/2020 (LEPABE)

Junta de Castilla y León y Fondo Europeo de Desarrollo Regional (FEDER) - (projects CLU 2017-09, UIC315 and VA266P20)

Junta de Castilla y León y Fondo Europeo de Desarrollo Regional (FEDER) - ((projects CLU 2017-09, CL-EI-2021-07, UIC315 and VA266P20)