Use of Specific Monoclonal Antibodies for Diagnosis of Citrus Tristeza Virus
1988
Vela, C. | Cambra, Mariano | Sanz, Antonio | Moreno, Pedro | Timmer, L. W.
Monoclonal antibodies (MAb) specific for citrus tristeza virus (CTV) were obtained by fusion of a nonsecreting myeloma cell line with spleen cells from immunized BALB/c mice. Immunoglobulin titre and isotype were determined in cell culture supernatants and mouse ascitic fluid. Each MAb was enzyme-conjugated and used in a double-antibody sandwich ELISA assay for CTV. All reacted uniformly against a panel of 23 strains of CTV representative of strains collected from Europe, Asia, and North America.
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