Genetic analysis of the tomato russet mite provides evidence of oligophagy and a widespread pestiferous haplotype
2023
Duarte, Mercia Elias | Lewandowski, Mariusz | de Mendonça, Renata Santos | Simoni, Sauro | Navia, Denise | Federal University of Piauí | Warsaw University of Life Sciences (SGGW) | Universidade de Brasilia = University of Brasilia [Brasília] (UnB) | Faculdade de Agronomia e Medicina Veterinária ; Universidade de Brasilia = University of Brasilia [Brasília] (UnB) | Consiglio per la Ricerca in Agricoltura e l’analisi dell’economia agraria = Council for Agricultural Research and Economics (CREA) | Research Centre for Plant Protection and Certification ; Consiglio per la Ricerca in Agricoltura e l’analisi dell’economia agraria = Council for Agricultural Research and Economics (CREA) | Centre de Biologie pour la Gestion des Populations (UMR CBGP) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD [Occitanie])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier ; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM) | To Foundation for Research Support of the Federal District (FAPDF), Brazil for the fellowship to the first author (MED) (PDJ. Process No. 193.001.418./2016). To the National Council for Scientific and Technological Development (CNPq), Brazil, for granting the fellowship to the first author (PDJ. Process No 158161/2018-1; DTI-A. Process No. 380382/2020-2 and Process No. 381542/2020-3 and DCR. Process No. 313349/2022-3) and last author (DN) (PP. Process No. 3121322017-4). To the Brazilian Coordination for the Improvement of Higher Education Personnel (CAPES), National Program of Post-Doctoral in Agronomy, Brazil for the financial support of RSM (CAPES-PNPD/Agronomy Proc. No. 88882.305808/2018-1). To Ministry of Agricultural, Food and Forestry Policies (MIPAAF) and Council for Agricultural Research and Economics (CREA), Italy, for funding RETIBIO (Ministerial Decree 92606, 22/12/2014) and RETIBIO II (Ministerial Decree 95785, 22/12/16). Part of the study was conducted by authors MED, RSM and DN at the Laboratory of Plant Quarantine, EMBRAPA Genetic Resources and Biotechnology, Brasilia, Brazil.
International audience
Afficher plus [+] Moins [-]anglais. Worldwide, the tomato russet mite (TRM), Aculops lycopersici (Eriophyidae), is a key pest on cultivated tomato in addition to infesting other cultivated and wild Solanaceae; however, basic information on TRM supporting effective control strategies is still lacking, mainly regarding its taxonomic status and genetic diversity and structure. As A. lycopersici is reported on different species and genera of host plants, populations associated with different host plants may constitute specialized cryptic species, as shown for other eriophyids previously considered generalists. The main aims of this study were to (i) confirm the TRM taxonomic unity of populations from different host plants and localities as well as the species' oligophagy, and (ii) to advance the understanding of TRM host relationship and invasion history. For this purpose, we evaluated the genetic variability and structure of populations from different host plants along crucial areas of occurrence, including the area of potential origin, based on DNA sequences of mitochondrial (cytochrome c oxidase subunit I) and nuclear (internal transcribed spacer, D2 28S) genomic regions. Specimens from South America (Brazil) and Europe (France, Italy, Poland, The Netherlands) were collected from tomato and other solanaceous species from the genera Solanum and Physalis. Final TRM datasets were composed of 101, 82 and 50 sequences from the COI (672bp), ITS (553bp) and D2 (605bp) regions, respectively. Distributions and frequencies of haplotypes (COI) and genotypes (D2 and ITS1) were inferred; pairwise genetic distance comparisons, and phylogenetic analysis were performed, including Bayesian Inference (BI) combined analysis. Our results showed that genetic divergences for mitochondrial and nuclear genomic regions from TRM associated with different host plants were lower than those observed in other eriophyid taxa, confirming conspecificity of TRM populations and oligophagy of this eriophyid mite. Four haplotypes (cH) were identified from the COI sequences with cH1 being the most frequent, representing 90% of all sequences occurring in all host plants studied (Brazil, France, The Netherlands); the other haplotypes were present exclusively in Brazilian populations. Six variants (I) were identified from the ITS sequences: I-1 was the most frequent (76.5% of all sequences), spread in all countries and associated with all host plants, except S. nigrum. Just one D2 sequence variant was found in all studied countries. The genetic homogeneity among populations highlights the occurrence of a highly invasive and oligophagous haplotype. These results failed to corroborate the hypothesis that differential symptomatology or damage intensity among tomato varieties and solanaceous host plants could be due to the genetic diversity of the associated mite populations. The genetic evidence, along with the history of spread of cultivated tomato, corroborates the hypothesis of a South American origin of TRM.
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