A Hopeful Sea-Monster: A Very Large Homologous Recombination Event Impacting the Core Genome of the Marine Pathogen Vibrio anguillarum
2020
Coyle, Nicola M | Bartie, Kerry L | Bayliss, Sion C | Bekaert, Michaël | Adams, Alexandra | McMillan, Stuart | Verner-Jeffreys, David W | Desbois, Andrew P | Feil, Edward J | BBSRC Biotechnology and Biological Sciences Research Council | University of Bath | Institute of Aquaculture | University of Bath | Institute of Aquaculture | Institute of Aquaculture | Institute of Aquaculture | CEFAS - Centre for Environment, Fisheries and Aquaculture Science | Institute of Aquaculture | University of Bath | 0000-0003-3037-5295 | 0000-0002-1206-7654 | 0000-0003-2368-7864 | 0000-0001-6052-8761
Vibrio anguillarum is the causative agent of vibriosis in many species important to aquaculture. We generated whole genome sequence (WGS) data on a diverse collection of 64 V. anguillarum strains, which we supplemented with 41 publicly available genomes to produce a combined dataset of 105 strains. These WGS data resolved six major lineages (L1-L6), and the additional use of multilocus sequence analysis (MLSA) clarified the association of L1 with serotype O1 and Salmonidae hosts (salmon/trout), and L2 with serotypes O2a/O2b/O2c and Gadidae hosts (cod). Our analysis also revealed a large-scale homologous replacement of 526-kb of core genome in an L2 strain from a con-specific donor. Although the strains affected by this recombination event are exclusively associated with Gadidae, we find no clear genetic evidence that it has played a causal role in host specialism. Whilst it is established that Vibrio species freely recombine, to our knowledge this is the first report of a contiguous recombinational replacement of this magnitude in any Vibrio genome. We also note a smaller accessory region of high single nucleotide polymorphism (SNP) density and gene content variation that contains lipopolysaccharide biosynthesis genes which may play a role in determining serotype.
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