Optimization of culture conditions for the production of exopolysaccharides from marine isolate <i>Citrobacter</i> sp.
2023
Pakeer Mohideen Askar Nawas | Seyed Mafiya Haniff | Karuppiah Sundararasu | Thiruvengadam Shankar | Sakthivel Venkatesh
Exopolysaccharide (EPS) samples were collected from the Mandapam camp, India. Morphological, biochemical characterization and 16S rRNA gene sequencing were done and the candidate bacterium was confirmed as <i>Citrobacter</i> sp. EPS production was observed from bacteria in Malt medium broth (0.88 ± 0.04mg/ml). The optimal cultural conditions for EPS production were as follows: after 72 hours of incubation at pH 8.0 (0.75 ± 0.1 mg/ml), the temperature at 40⁰C (0.75 ± 0.1 mg/ml), 100 rpm (0.90 ± 0.1 mg/ml). In the experimentation, arabinose (carbon sources) (1. ± 0.120 mg/ml) and ammonium nitrate (nitrogen) (1.30 ± 0.1 mg/ml) showed better results for the yield of EPS. The highest production of EPS was recorded in Triton X-100 (surfactants) supplemented medium. The optimal production of EPS was obtained in 2.5% sodium chloride added medium. Then the 2.5% inoculum concentration better for maximizing the yield of EPS. The best yield of EPS was noticed in after 96 hours of incubation time.
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