Habitat related variations and comparison of fatty acid composition of muscle and stomach contents of secondarily colonized Malabar Sprat (Ehirava fluviatilis) in Sri Lanka
2019
de Zoysa, H.K.S. | Amarasinghe, U.S. | Edirisinghe, E.M.R.K.B. | Sundarabarathy, T.V.
Ehirava fluviatilis is a marine origin clupeid found in Southern India and Sri Lanka, also found in two freshwater lakes, namely Parakarama Samudraya and Rajanaganaya reservoir, and that places are identified as secondarily colonizedhabitats. The objectives of this study were carried toinvestigate the fatty acid (FA) profile in the flesh of E.fluviatilis, to investigate the FA profile in the food of E. fluviatilis, and identify the impactof the FA profiles for colonisation. The Bligh and Dyer extracting method was used to lipids extraction of flesh and stomach contents of E. fluviatilis and Gas Chromatography (GC) was used to identify the different types of FAs. Data were statistically analyzed to examine the differences in the quantities of different FAs using ANOVA and principal component analysis (PCA). The predominant FAs were recorded in two different habitats ofE. fluviatilis in both flesh) and stomach contents. The only recorded difference between FAs profile was the presence of C 18:2 (n-4) in the Rajanganaya reservoir. The difference in the FA profile may lead to colonization of marine origin E. fluviatilis in Rajanganaya reservoir as a freshwater lacustrine habitat due to the ability of modification of FAs. Further, this study also indicates that the presence of specific FAs could be used as biomarkers to identify species.
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