Highly polymorphic microsatellite markers for the short-snouted seahorse (hippocampus hippocampus), including markers from a closely related species the long-snouted seahorse (hippocampus guttulatus)
2009
Van De Vliet, M. S. | Diekmann, O. E. | Serrão, Ester
Species of the family Syngnathidae are increasingly being investigated for conservation purposes but also for mating system and genetic parentage studies. The availability of highly polymorphic markers will be of great importance to conduct these kinds of studies. This paper describes the development and characterization of 10 polymorphic microsatellite markers for Hippocampus hippocampus and the utility of nine markers which were developed for a closely related species Hippocampus guttulatus. The number of alleles per locus developed for H. hippocampus ranged from 14 to 30, with levels of heterozygosity between 0.90 and 0.95. Most of the markers were successfully cross-amplified in the sister species, giving clear and unambiguous peaks and maintaining high levels of polymorphism.
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