Phenol Removal from Wastewater with Horseradish Peroxidase Immobilized by Periodate Method Onto Novel Macroporous Poly(gma-Co-Egdma) Carriers
2024
Surudžić, Nevena | Spasojević, Milica | Crnoglavac Popović, Milica | Stanišić, Marija | Prodanović, Radivoje | Prodanović, Olivera
One of the most important environmental problems that need to be adressed is wastewater contamination. Numerous reserarches have focused on solving the aforementioned problem by applying immobilized enzymes. When horseradish peroxidase, the most commonly used enzyme, is immobilized by one of many available methods on a suitable support, a biocatalyst with improved properties in terms of increased stability and reusability, is obtained. Dispersion polymerization was used for the synthesis of macroporous poly(GMA-co-EGDMA) copolymers with different pore size diameters. Prior to immobilization of HRP onto these carriers, their amination with ethylenediamine was performed. Periodate method was applied for the formation of covalent bond between the enzyme and used copolymer. Increase in specific activity of immobilized peroxidase was noticed. The copolymer with the pore size diameter of 297 nm showed the highest activity.
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Editeur University of Belgrade, Technical faculty in Bor (Serbia)
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