The Effect of Low Intensity Exercise on Expression of Inflammatory Response and Apoptosis in Rats with Obesity Induced by a High-Fat Diet
2018
Dong-Hun Choi | Joon-Yong Cho
PURPOSE The goal of this study is to demonstrate whether low intensity exercise has an effect on the level of inflammatory response and apoptosis in rats with obesity induced by a high-fat diet. METHODS A high fat diet (60% fat, 20% carbohydrate, and 20% protein) was treated to a subset of experimental rats for 35 weeks to induce obesity before exercise intervention. The rats were randomly assigned into 3 groups: normal control (ND-CON, n = 7), High-Fat Diet control (HFD-CON, n=7) and High-Fat Diet+Exercise (HFD-EXE, n=7).The low intensity exercise program was performed for 10 weeks with treadmill (30 min/day, 5 days/week). After treadmill exercise, body weight, total fat mass, HOMA-IR and OGTT were analyzed. Also TLR-4 signaling pathway related proteins, inflammation related factors and apoptosis were analyzed using Western blot method. RESULTS Low intensity exercise with treadmill reduces body weight and body fat of obese rats that were induced by high fat diet, and improves insulin resistance and blood glucose recovery ability. In addition, low intensity exercise inhibits the activity of NF-κB by reducing TLR4 protein, which regulates inflammation and immunity. This has shown to reduce the inflammatory cytokines IL-1β and TNF-α, Bax and Caspase3 proteins associated with apoptosis, and improve Bcl2 protein. CONCLUSIONS In conclusion, low intensity exercise reduces the level of TLR4 protein that regulates immune and inflammatory responses in adipocytes of obese rats induced by high fat diet. This inhibits the activity of inflammatory cytokines and apoptosis-related proteins, which will be effective in the prevention and treatment of obesity.
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