冷压初榨椰子油对四氯化碳致小鼠 急性肝损伤的保护作用Hepatoprotective effects of virgin cold-pressed coconut oil against carbon tetrachloride induced acute liver injury in mice

研究了冷压初榨椰子油对四氯化碳（CCl4）诱导昆明小鼠急性肝损伤的保护作用。将72只昆明小鼠随机分成6组，包括正常组、四氯化碳肝损伤模型组、联苯双酯组（150 mg/kg）、椰子油低剂量组（5 mL/kg）、椰子油中剂量组（10 mL/kg）、椰子油高剂量组（20 mL/kg），通过测定小鼠血清中丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、乳酸脱氢酶（LDH）、甘油三酯（TG）和总胆固醇（TC）指标，肝组织中超氧化物歧化酶（SOD）活力、谷胱甘肽过氧化物酶（GSH-Px）活力及丙二醛（MDA）含量，采用HE染色切片对小鼠肝脏组织进行病理学检查，考察冷压初榨椰子油对四氯化碳致小鼠急性肝损伤的保护作用。结果表明：与模型组相比，不同剂量组的冷压初榨椰子油（5、10 mL/kg和20 mL/kg）可以不同程度地降低小鼠血清中ALT、AST、LDH、TG、TC水平以及肝组织中MDA含量，提高肝组织中SOD活力与GSH-Px活力；同时，肝脏组织病理学观察与生化指标检测结果一致。其中，高剂量的冷压初榨椰子油(20 mL/kg)作用效果优于阳性对照联苯双酯。研究表明冷压初榨椰子油对肝脏具有较好的保护作用，作用机制可能与其含有较多的抗氧化物质以及功能性物质有关。The hepatoprotective effects of virgin cold-pressed coconut oil (VCPCO) against carbon tetrachloride (CCl4) induced acute liver injury in Kunming (KM) mice were studied. A total of 72 Kunming mice were randomly divided into 6 groups, including normal group, CCl4 liver injury model group, bifendate group (150 mg/kg), coconut oil low dosage group (5 mL/kg), coconut oil medium dosage group (10 mL/kg) and coconut oil high dosage group (20 mL/kg). The levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), triglyceride (TG), total cholesterol (TC) in serum, and superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) in liver were determined. The liver tissue was examined pathologically by HE staining section. The results showed that VCPCO with dosages of 5, 10 mL/kg and 20 mL/kg could decrease the levels of ALT, AST, LDH, TG, TC in serum and MDA in liver, and increase the levels of SOD and GSH-Px in liver. Meanwhile, the results of liver histopathology were consistent with those of biochemical markers. Among them, the effect of high dosage of VCPCO (20 mL/kg) was better than the positive control bifendate. Results indicated that VCPCO had a good protective effect on liver, and the mechanism might be related to its high content of antioxidative and functional compounds.