Effects of <i>TaMTL</i>-Edited Mutations on Grain Phenotype and Storage Component Composition in Wheat
2022
Huali Tang | Shuangxi Zhang | Mei Yu | Ke Wang | Yang Yu | Yuliang Qiu | Yanan Chang | Zhishan Lin | Lipu Du | Daolin Fu | Zichao Li | Xingguo Ye
Wheat nutrition and processing-quality are primarily based on the endosperm ingredients. However, the effect of embryos on grain traits and components remains unclear. In this study, we found that in the cross-pollinated and self-pollinated progenies of the four wheat <i>mtl</i> mutants (<i>mtl</i>-<i>A</i>, <i>mtl</i>-<i>AD</i>, <i>mtl</i>-<i>BD</i>, and <i>mtl</i>-<i>ABD</i>) the haploid induction rates were 0–15.6% and 0–14.1%, and the embryo abortion rates were 0–27.4% and 0–24.1%, respectively, in which <i>mtl-A</i> had no effect on haploid induction and embryo development. The embryoless grains (ELG) were comparable to the normal grains (NG) from <i>mtl</i>-<i>AD</i>, <i>mtl</i>-<i>BD</i>, and <i>mtl</i>-<i>ABD</i> in grain length, grain width and thousand-kernel weight, but the grain traits were significantly less than those in NG from mutant <i>mtl-A</i>. During grain filling period, <i>mtl-ABD</i> had similar ELG ratio and amount of starch granule (SG) and protein body (PB) in ELG and NG. At maturity stage, the morphological features of A-type and B-type SG in ELG were similar to those in NG in <i>mtl</i> mutants; however, amylose, gliadin, and glutenin contents were higher in ELG, and total starch, albumin and globulin contents were higher in NG. Our results clarified the effect of the wheat <i>mtl</i> mutants on haploid induction and grain traits and nutrition composition in this crop, and provided new clues for studying the development of embryo and endosperm and their interaction in plants.
Afficher plus [+] Moins [-]Informations bibliographiques
Cette notice bibliographique a été fournie par Directory of Open Access Journals
Découvrez la collection de ce fournisseur de données dans AGRIS