Determination of the viability of Azospirillum syrups, multiplied in different culture media
2023
Gabriel Fleck da Rosa | Cristina Rossetti | Priscila Erbice Olin | Yago Müller Alves | Andreia da Silva Almeida
The use of products of microbial origin, bacteria, for agriculture has grown exponentially in the world, and in Brazil this growth is significant. In recent years, several companies have settled in the country and started to develop different products in this segment. The producer has the possibility of multiplying bacteria on the farms themselves in a process called “on Farm”, a homemade way of multiplying bacteria. The objective of this work was to evaluate the efficiency of the mixtures produced through the “on Farm” method of multiplication of microorganisms from inoculants based on Azospirillum with different culture media. For this, three culture media were used: commercial product Multibacter®, yeast syrup plus sugar and yeast syrup, which were subsequently quantified. The syrups were produced in the on Farm system, the sterilization of the place of packaging and allocation of the bioreactors was carried out, the samples of the syrups were diluted for incubation and the preparation of the TSA also took place. It was possible to conclude that the non-sterile culture media do not present satisfactory results for the multiplication of on Farm microorganisms with Azospirillum syrups. Colony forming units are not parameters to indicate viability for non-sterile culture media.
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