Acclimatization of terrestrial orchid Bletilla striata Rchb.f. (Orchidaceae) propagated under in vitro conditions
2012
Helena LESAR | Barbara HLEBEC | Nataša ČERANIČ | Damijana KASTELEC | Zlata LUTHAR
Bletilla striata is a terrestrial sympodial orchid. Substrates used for outdoor growing, differing in the mixture of added components and nutrients, were chosen for acclimatization of the asymbiotic propagated plants. A total of 651 Bletilla striata orchids were planted in 3 commercial substrates: ″Tonsubstrat″ (Ton), ″Baltisches substrat″ (Baltski) and ″Royal-Garden″ (Royal). Prior to acclimatization, the plants were 2.5 cm on average, with at least 2 leaves and 2 - 3 cm long root or roots. Fewest plants (3.1%) died in Ton substrate, 3.2% in Baltski substrate and 5.0% in Royal substrate. There were no statistically significant differences among substrates (p = 0.558) in the percentage of plants that died. Substrates used in combination with the chosen phenophase and established conditions were suitable for acclimatization of Bletilla striata orchids, whereby 95-97% of plants successfully adapted from heterotrophic to autotrophic conditions in a very short period of two months. The basic conditions for success are that plants are large and vital enough prior to acclimatization, that the substrate is appropriate and that appropriate conditions of relative humidity, temperature, light and ventilation without major fluctuations of these factors are ensured during acclimatization.
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