Genus-Wide Microsatellite Primers for the Goldenrods (<i>Solidago</i>; Asteraceae)
2014
James B. Beck | John C. Semple | Justin M. Brull | Stacey L. Lance | Mai M. Phillips | Sara B. Hoot | Gretchen A. Meyer
Premise of the study: Microsatellite primers were developed for studies of polyploid evolution, ecological genetics, conservation genetics, and species delimitation in the genus <i>Solidago</i>. Methods and Results: Illumina sequencing of a shotgun library from <i>S. gigantea</i> identified ca. 1900 putative single-copy loci. Fourteen loci were subsequently shown to be amplifiable, single-copy, and variable in a broad range of <i>Solidago</i> species. Conclusions: The utility of these markers both across the genus and in herbarium specimens of a wide age range will facilitate numerous inter- and intraspecific studies in the ca. 120 <i>Solidago</i> species.
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