QTL Detection of milk, fat yeild and percentage on chromosome 14 in Iranian Holstein cattle population using microsatellite markers
2014
Mahnaz Nouri Sadeq | Morad Pasha Eskandari Nasab | Rahim Asfouri | Saeid Ansari Mahyari | Mohammad Taher Haraki Nezhad
This study was carried out to detect QTLs affecting milk production traits in Iranian Holstein population using 10 microsatellite markers including: ILSTS039, CSSM066, ILSTS011, CBDIKM002, CBDIKM004, DIK5080, DIK4884, DIK4361, DIK258 and BM1508. Samples were collected from 10 sire families including 233 milking cows from dairy farms of ten provinces in Iran (Isfahan, Central province, Khorasan, Fars, Yazd, Qazvin and Zanjan). Genomic DNA extracted using standard kit. Genotyping of the samples was performed with capillary electrophoresis. Genotypic and phenotypic data were statistically analyesed using DMU software. Most probable places for detecting QTL in which LRT was meaningful at 5% for milk fat percentage were observed at 3 cM, for milk production at 54 cM and also QTL of milk fat production was detected in the position 54 cM on chromosome 14.
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