Occurrence and genetic characterization of Staphylococcus aureus in milk samples of cattle with mastitis, and in the Veterinary Hospital personnel and dairy workers

Lucianne Leigue; Ayrton Rodrigo Hilgert; Adriana Fiorini; Marise Fonseca dos Santos; Eliane Cristina Gruzka Vendruscolo

Occurrence and genetic characterization of Staphylococcus aureus in milk samples of cattle with mastitis, and in the Veterinary Hospital personnel and dairy workers

2017

Lucianne Leigue | Ayrton Rodrigo Hilgert | Adriana Fiorini | Marise Fonseca dos Santos | Eliane Cristina Gruzka Vendruscolo

Staphylococcus aureus is one of the most common microorganisms responsible for high morbidity and mortality in humans and animals. Methicillin-resistant S. aureus are responsible for several outbreaks worldwide and therapeutic arsenal has become scarce. The present investigation verified the epidemiological profile of S. aureus strains isolated from the veterinary hospital staff, from dairy cattle workers and also from milk samples of dairy cattle presenting mastitis. Samples were characterized phenotypically by antibiogram, catalase, and coagulase tests, and also by Voges-Proskauer test. The isolated strains were characterized genotypically by specific Polymerase Chain Reaction and Amplified Ribosomal DNA Restriction Analysis (ARDRA). From the 218 isolated strains, 27 were identified as S. aureus (12%), four of them were resistant to oxacillin and two of them were classified as MRSA (Methicillin-resistant S. aureus). The prevalence of isolated strains among animal personnel care was low (2%) but all MRSA isolates were found among the clinical staff. Results of ARDRA pointed out that S. aureus strains isolated from different animal care personnel were grouped in the same cluster when HindIII and HinfII restriction enzymes were used. When ARDRA was performed with HaeIII enzyme, the formation of two clusters was observed, but the isolated strains were not correlated. The prevalence of S. aureus strains isolated was higher in clinical staff and the biochemical and molecular assays of them presented 100% of correlation.

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