Guidelines for DNA recombination and repair studies: Mechanistic assays of DNA repair processes

Hannah L Klein; Kenny K.H. Ang; Michelle R. Arkin; Emily C. Beckwitt; Yi-Hsuan Chang; Jun Fan; Youngho Kwon; Michael J. Morten; Sucheta Mukherjee; Oliver J. Pambos; Hafez el Sayyed; Elizabeth S. Thrall; João P. Vieira-da-Rocha; Quan Wang; Shuang Wang; Hsin-Yi Yeh; Julie S. Biteen; Peter Chi; Wolf-Dietrich Heyer; Achillefs N. Kapanidis; Joseph J. Loparo; Terence R. Strick; Patrick Sung; Bennett Van Houten; Hengyao Niu; Eli Rothenberg

Guidelines for DNA recombination and repair studies: Mechanistic assays of DNA repair processes

2019

Genomes are constantly in flux, undergoing changes due to recombination, repair and mutagenesis. In vivo, many of such changes are studies using reporters for specific types of changes, or through cytological studies that detect changes at the single-cell level. Single molecule assays, which are reviewed here, can detect transient intermediates and dynamics of events. Biochemical assays allow detailed investigation of the DNA and protein activities of each step in a repair, recombination or mutagenesis event. Each type of assay is a powerful tool but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies.

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Mots clés AGROVOC

homologous recombination

Informations bibliographiques

Publié dans

Microbial Cell

Volume 6 Numéro 1 Pagination 65 - 101 ISSN 2311-2638

Editeur

Shared Science Publishers OG

D'autres materias

Endonuclease protection assay; Dna repair centers; Single-molecule; Dna helicases; Recombinase filament assembly; Mismatch repair; Fluorescent proteins; Dna repair synthesis; Transcription coupled repair; Dna resection; Single-particle tracking; Crossovers; Genome instability; Synthesis-dependent strand annealing; Nucleotide excision repair; Dna breaks; Photoactivated fluorescent proteins; Chromatin dynamics; Nonhomologous end joining; Dsbs; Double strand break repair; Super resolution; Palm; Chromosome rearrangements; Gross chromosome rearrangements; Fret; Structure-selective endonucleases

Langue

anglais

Sur AGRIS depuis: 2024-12-11

Date de modification: 2025-03-19

Format: DOAJ

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Liens

DOI http://microbialcell.com/researcharticles/2019b-klein-microbial-cell/

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