Visualizing the ribonucleoprotein content of single bunyavirus virions reveals more efficient genome packaging in the arthropod host
2021
Erick Bermúdez-Méndez | Eugene A. Katrukha | Cindy M. Spruit | Jeroen Kortekaas | Paul J. Wichgers Schreur
Bermúdez-Méndez et al. describe a FISH-immunofluorescence method that allows simultaneous visualization of bunyavirus genome segments and virions at single-molecule resolution. They show that the packaging of bunyavirus genome segments into virions is a flexible, non-selective process and that genome packaging is more efficient in insect cells compared to mammalian cells.
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