Discovery and modification of cytochrome P450 for plant natural products biosynthesis
2020
Xiaonan Liu | Xiaoxi Zhu | Hui Wang | Tian Liu | Jian Cheng | Huifeng Jiang
Cytochrome P450s are widespread in nature and play key roles in the diversification and functional modification of plant natural products. Over the last few years, there has been remarkable progress in plant P450s identification with the rapid development of sequencing technology, “omics” analysis and synthetic biology. However, challenges still persist in respect of crystal structure, heterologous expression and enzyme engineering. Here, we reviewed several research hotspots of P450 enzymes involved in the biosynthesis of plant natural products, including P450 databases, gene mining, heterologous expression and protein engineering.
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