Isolation and Phenotypic Microarray Profiling of Different <i>Pseudomonas</i> Strains Isolated from the Rhizosphere of <i>Curcuma longa</i> L.
2023
Parul Pathak | Monika Singh | Ananya Naskar | Sandeep Kumar Singh | Nikunj Bhardwaj | Ajay Kumar
In the present study, different <i>Pseudomonas</i> strains were isolated from the rhizospheric soil of <i>Curcuma longa</i> (turmeric) and further identified and characterized based on morphological, biochemical, and molecular characteristics through the 16S rRNA gene sequencing analysis. The identified bacterial strains belong to the <i>Pseudomonas</i> genus viz. <i>Pseudomonas</i> sp. CL10, <i>Pseudomonas</i> sp. CL11, and <i>P. fluorescence</i> CLI4. However, the isolated strains tested positive for IAA production, siderophore production, and the solubilization of tricalcium phosphate during plant growth promoting traits analysis. Further phenotype microArray (PM) technology was used to evaluate the antibiotic and chemical sensitivity of the isolated bacterial strains. The antibiotics phleomycin, oxacillin, vancomycin, novobiocin, spiramycin, and rifampicin, as well as chemicals like, 5-7 dichloro-8-hydroxy quanaldine, 5-7 dichloro-8-hydroxyquinoline, domophenbrobide, and 3-5 dimethoxy benzyl alcohol, showed resistance in all the rhizobacterial strains. However, upon further detailed study, <i>Pseudomonas</i> sp. CL10 exhibited resistance to thirteen antibiotics, CL11 to fourteen, and CL14 showed resistance against seventeen antibiotics and chemical classes. The results of the study indicate that some of these strains can be used as bioinoculum to enhance the plant growth and health.
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