Phyto-Synthesis, Characterization, and In Vitro Antibacterial Activity of Silver Nanoparticles Using Various Plant Extracts
2022
Bilal Ahmad | Li Chang | Usama Qamar Satti | Sami ur Rehman | Huma Arshad | Ghazala Mustafa | Uzma Shaukat | Fenghua Wang | Chunyi Tong
<i>Aloe vera</i>, <i>Mentha arvensis</i> (mint), <i>Coriandrum sativum</i> (coriander), and <i>Cymbopogon citratus</i> (lemongrass) leaf extracts were used to synthesize stable silver nanoparticles (Ag-NPs) by green chemistry. UV–vis spectrophotometry, X-ray diffraction (XRD), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and energy dispersive X-ray (EDX) spectroscopy techniques were used to characterize these biosynthesized nanoparticles. The data indicated that the silver nanoparticles were successfully synthesized, and the narrower particle size distribution was at 10–22 nm by maintaining a specific pH. As a short-term post-sowing treatment, Ag-NP solutions of different sizes (10 and 50 ppm) were introduced to mung bean seedlings, and the overall increase in plant growth was found to be more pronounced at 50 ppm concentration. The antibacterial activity of Ag-NPs was also investigated by disc diffusion test, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) test. The zones of inhibition (ZOI) were shown by <i>Escherichia coli</i> (<i>E. coli</i>) (1.9, 2.1, 1.7, and 2 mm), followed by <i>Staphylococcus aureus</i> (<i>S. aureus</i>) (1.8, 1.7, 1.6, and 1.9 mm), against coriander, mint, <i>Aloe vera</i>, and lemongrass, respectively. MIC and MBC values of <i>E. coli,</i> and <i>S. aureus</i> ranged from 7 to 8 µg/mL. Overall, this study demonstrates that Ag-NPs exhibit a strong antimicrobial activity and thus might be developed as a new type of antimicrobial agent for the treatment of bacterial infection.
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