Diagnosis of the primary infection by pneumocystis in autopsy specimens from two infants using lung impression smears (touch preps)
2014
Carolina A. Ponce | Rebeca I. Bustamante | Myriam Gallo | Sergio L. Vargas
The primary infection by Pneumocystis of normal, healthy infants is asymptomatic and goes undiagnosed. Microscopy diagnosis of Pneumocystis was sought in lung impression smears (LIS) from two ~3-month-old infants dying unexpectedly in the community. Pneumocystis nuclei and cysts were identified using Hema-Gurr with subsequent Gomori–Grocott staining in the same spot documenting that these stains may be complementary. LIS provide for an observer–dependent, inexpensive, and ready-available method for detection of Pneumocystis in infant lungs.
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