Refining isoflavones from Puerariae flos and its in vitro lipid-lowering activity
2022
WU Wei | LI Qun | SONG Di | SUN Ming-zhe
<b>Objective:</b> The refining parameters of isoflavones from <i>Puerariae flos</i> were optimized by using HPD-100 macroporous adsorption resin, and its <i>in vitro</i> lipid-lowering effects were evaluated. <b>Methods:</b> The optimized refine parameters of isoflavones from <i>Puerariae flos</i> were studied by macroporous adsorption resin, and the lipid-lowering effects of isoflavones from <i>Puerariae flos</i> were evaluated by pancreatic lipase enzyme activity, pancreatic cholesterol esterase enzyme activity and micellar cholesterol solubility. <b>Results:</b> Adsorption parameters: <i>Puerariae flos</i> isoflavones were adsorbed with concentration of 100 mg/mL, pH 6.0 and upper column volume of 20 mL. Desorption parameters: the adsorption resins were washed by 70% ethanol with pH 5.0 at flow rate of 2.0 mL/min. The purity of refined <i>Puerariae flos</i> isoflavones were 81.47%. The inhibition ratio on pancreatic lipase enzyme, pancreatic cholesterol esterase enzyme and micellar cholesterol solubility were 40.73%, 52.32% and 46.17%, respectively, and these were increased by 26.65%, 47.46% and 37.53% respectively, compared with not refining. <b>Conclusion:</b> <i>Puerariae flos</i> isoflavones showed lipid-lowering effects by inhibiting pancreatic lipase enzyme activity, pancreatic cholesterol esterase enzyme activity and micellar cholesterol solubility.
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