A convenient method for simultaneous quantification of multiple phytohormones and metabolites: application in study of rice-bacterium interaction
2012
Liu Hongbo | Li Xianghua | Xiao Jinghua | Wang Shiping
<p>Abstract</p> <p>Background</p> <p>Simultaneous analysis of multiple functional-related phytohormones and their metabolites will improve our understanding of interactions among different hormones in the same biologic process.</p> <p>Results</p> <p>A method was developed for simultaneous quantification of multiple phytohormones, abscisic acid, indole-3-acetic acid (IAA), jasmonic acid (JA), and salicylic acid, hormone conjugates, IAA-aspartic acid, JA-isoleucine, and methyl JA, and phytoalexins, momilactone A, naringenin, and sakuranetin. This method combines a convenient procedure for preparing filtrated crude extracted samples and a sensitive quantification assay using ultra fast liquid chromatography-electrospray ionization tandem mass spectrometry (UFLC-ESI-MS). With this method, we determined the dynamic profiles of defense-related phytohormones, hormone metabolites, and phytoalexins in the interaction of rice with <it>Xanthomonas oryzae </it>pv. <it>oryzae </it>(<it>Xoo</it>), which causes bacterial blight, one of the most devastating diseases of rice worldwide.</p> <p>Conclusion</p> <p>This UFLC-ESI-MS method is convenient, sensitive, reliable, and inexpensive for quantification of multiple phytohormones and metabolites compared to current methods. The results obtained by application of this method in studying rice-bacterial interaction provide a basis for understanding the molecular mechanisms of rice defense responses.</p>
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