Molecular analysis of Cryptosporidium species in domestic goat in central Iraq
2022
Akeel M. Al-Musawi | Abdul Hussein H. Awad | Mansoor J. Alkhaled
<em>Cryptosporidium</em> spp. is a significant parasitic disease that results in diarrhea and gastroenteritis in humans and animals worldwide. The present study aimed to investigate the molecular diversity of <em>Cryptosporidium</em> species in domestic goats. A total of a hundred feces samples were collected from four locations in Babylon city in central Iraq. All the samples were investigated phenotypically using a modified Ziehl-Neelsen stain method and genotypically using conventional and nested PCR methods based on a partial sequence of heat shock protein 70 (Hsp70) and 60 kDa glycoprotein (gp60) genes, and finally, phylogenetic analysis method. The molecular results showed five species of <em>Cryptosporidium</em>, including <em>C. parvum, C. hominis, C. ryana, C. xiaoi, </em>and<em> C. bovis</em>. The phylogenetic results of partial sequence of gp60 gene for <em>C. parvum</em> and C. hominis isolate two subtypes were established IIdA21G1 and IIdA19G1 belong to <em>C. parvum</em>. For <em>C. hominis</em>, three subtypes were detected: IbA21G2, IbA13G3, and IbA19G2. This study showed that <em>Cryptosporidium parvum </em>(zoonotic) is more prevalent than other <em>Cryptosporidium</em> species in goats from this area. This suggests that zoonotic transmission is the primary mode of transmission of <em>Cryptosporidium</em> infection in Babylon province.
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