Isolation and molecular-based identification of bacteria from unhatched leftover eggs of ducks in selected mini-hatcheries of Kishoreganj, Bangladesh

Sadia Afrin Punom; Md. Shahidur Rahman Khan; Shayka Tasnim Pritha; Jayedul Hassan; Saifur Rahman; Md. Muket Mahmud; Md. Shafiqul Islam

Isolation and molecular-based identification of bacteria from unhatched leftover eggs of ducks in selected mini-hatcheries of Kishoreganj, Bangladesh

2020

Objectives: The study was designed for isolation and identification of the bacteria present in unhatched leftover eggs of duck in selected mini-hatcheries of Kishoreganj, Bangladesh. Materials and Methods: A total of 54 unhatched discarded eggs were collected as samples from different mini-hatcheries of Tarail and Itna Upazilas of Kishoreganj and aseptically carried to the laboratory in the icebox. Surface washings (n = 54) and inner contents (n = 54) were collected and enriched in LuriaBertani broth followed by the isolation of pure colonies of different bacte¬ria onto eosin methylene blue agar, mannitol salt agar, SalmonellaShigella agar, and blood agar plates. Identification of the bacterial isolates was done by cultural properties, staining, and bio¬chemical tests followed by molecular detection by Polymerase chain reaction. Results: Of 108 samples, 62 were found positive for Salmonella spp. (76%), 59 for E. coli (54%), 52 for Staphylococcus spp. (48%), and 5 for Clostridium spp. (9%). From the egg surface samples, Staphylococcus spp. were recovered in the highest (67%) followed by Salmonella spp. (59%), E. coli (56%), and Clostridium spp. (9%). From the inner contents of eggs, Salmonella spp. were recovered in the highest (56%), followed by E. coli (53%) and Staphylococcus spp. (30%). Conclusion: The isolated bacteria might be associated with the decreased hatchability and embryo mortality in the mini-hatcheries of duck. [J Adv Vet Anim Res 2020; 7(1.000): 164-169]

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