Toxicopathological and biochemical effects of Carbon Tetrachloride CCl4 with residual accumulation in Liver of mice

Objective :-  The aims of the present study are to determine the toxicopathological and pathophysiological effects of carbon tetrachloride (CCl4), with measurement residual quantity of CCl4 in liver tissue by (HSGC) method.     Methodology :- 40 Swiss strain white mice were used, average weigh about 30 –32 g , divided into three groups, the T1 and T2 group were administrated with (100 and 200 mg/kg/body weight CCl4 respectively , given via intraperitoneal injection two does weekly for 40 day). While the 3rd group served as control. Clinical signs were reported during the course of the study, then at day 40 post treatment , all animal were sacrificed, blood were collected at 20 and 40 days of treatment period for biochemical test  and post mortem examination was done and any gross lesions were reported, liver section was taken for pathophysiological examination, also for measurement residual accumulated in tissue by Head-Space Gas Chromatographic analysis (HSGC) method.     Results :- All results showed that CCl4 caused significant increase of serum alanine aminotransferase and aspartate aminotransferase activity. Histopathological picture of liver, showed damage of liver parenchyma, disappear arrangements of hepatocyte, loss of hepatic cord, with congestion and dilatation of blood vessel also coagulative necrosis and apoptotic cells. Infiltration of inflammatory cells (macrophages &lymphocyte). Also high quantity from CCl4 was residual in hepatic tissue.     Conclusion :- The present study investigated that the CCl4 affected on the liver tissue, and causes histopathological lesion with elevation of liver enzymes specially ALT& AST , with accumulative effect in liver tissue after measurement by GC system. And the degree of influence depended on the concentration of the toxic dose .